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12 questions to help you make sense of cohort study – Critical Appraisal Skills Programme

(A) Are the results of the study valid?

Screening Questions

1. Did the study address a clearly focused issue?
HINT: A question can be ‘focused’ In terms of

  • The population studied
  • The risk factors studied
  • The outcomes considered
  • Is it clear whether the study tried to detect a beneficial
    or harmful effect?

Yes Can’t tell No

2. Was the cohort recruited in an acceptable way?
HINT: Look for selection bias which might compromise the generalisability of the findings:

  • Was the cohort representative of a defined population?
  • Was there something special about the cohort?
  • Was everybody included who should have been included?

Yes Can’t tell No

Is it worth continuing?

Detailed questions

3. Was the exposure accurately measured to minimise bias?
HINT: Look for measurement or classification bias:

  • Did they use subjective or objective measurements?
  • Do the measurements truly reflect what you want them to (have they been validated)
  • Were all the subjects classified into exposure groups using the same procedure

Yes Can’t tell  No

4. Was the outcome accurately measured to minimise bias?
HINT: Look for measurement or classification bias:

  • Did they use subjective or objective measurements?
  • Do the measures truly reflect what you want them to (have they been validated)?
  • Has a reliable system been established for detecting all the cases (for measuring disease
    occurrence)?
  • Were the measurement methods similar in the different groups?
  • Were the subjects and/or the outcome assessor blinded to exposure (does this matter)?

Yes Can’t tell  No

5. (a) Have the authors identified all important confounding factors?

List the ones you think might be
important, that the author missed.

Yes Can’t tell  No

(b) Have they taken account of the confounding factors in the design and/or analysis?
HINT: Look for restriction in design, and techniques e.g. modelling, stratified-, regression-, or sensitivity analysis to correct, control or adjust for confounding factors

Yes Can’t tell  No

6. (a) Was the follow up of subjects complete enough?

Yes Can’t tell  No

(b) Was the follow up of subjects long enough?
HINT: Consider

  • The good or bad effects should have had long enough to reveal themselves
  • The persons that are lost to follow-up may have different outcomes than those available for assessment
  • In an open or dynamic cohort, was there anything special about the outcome of the people leaving, or the exposure of the people entering the cohort?

Yes Can’t tell  No

(B) What are the results?

7. What are the results of this study?
HINT: Consider

  • What are the bottom line results?
  • Have they reported the rate or the proportion between the exposed/unexposed, the ratio/the rate difference?
  • How strong is the association between exposure and outcome (RR,)?
  • What is the absolute risk reduction (ARR)?

Yes Can’t tell No

8. How precise are the results?
HINT: Look for the range of the confidence intervals, if given.

Yes Can’t tell No

9. Do you believe the results?
HINT: Consider

  • Big effect is hard to ignore!
  • Can it be due to bias, chance or confounding?
  • Are the design and methods of this study sufficiently flawed to make the results unreliable?
  • Bradford Hills criteria (e.g. time sequence, dose-response gradient, biological plausibility, consistency)

Yes Can’t tell No

(C) Will the results help locally?

10. Can the results be applied to the local population?
HINT: Consider whether

  • A cohort study was the appropriate method to answer this question
  • The subjects covered in this study could be sufficiently different from your population to cause concern
  • Your local setting is likely to differ much from that of the study
  • You can quantify the local benefits and harms

Yes Can’t tell No

11. Do the results of this study fit with other available evidence?

Yes Can’t tell No

12. What are the implications of this study for practice?
HINT: Consider

  • One observational study rarely provides sufficiently robust
    evidence to recommend changes to clinical practice or
    within health policy decision making
  • For certain questions observational studies provide the only
    evidence
  • Recommendations from observational studies are always stronger
    when supported by other evidence

Yes Can’t tell No

12 questions to help you make sense of cohort study – Critical Appraisal Skills Programme
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