Investigation of Complement Function | My Assignment Tutor

2020-21 BHS002-2 / BHS027-2 Immunology Dr Anna FurmanskiInvestigation of Complement Function (CH50 assay)You will analyse results obtained from tests performed in the immunology laboratory and will reporton your conclusions. Serum samples from two patients were tested along with a Reference serum(healthy control) in CH50 assays to determine serum complement function.Patient history:Patient X:• 19 year old male• Symptoms include: fever, headache, confusion, somnolence• No evidence of recent respiratory tract infection, patient is HIV-negative• Patient has a sister who has had two unusual prior episodes of significant Neisseriameningitidis infectionsPatient Y:• 30 year old male• Symptoms include: persistent and worsening urticaria, angioedema (of lips), and palpablepurpura on torso consistent with vasculitis• Patient showed blood markers consistent with renal inflammationData generated from CH50 assaysUse, analyse and report on the data below in preparing your lab report. You may copy/paste thistable without referencing it. Please consider whether, rather than directly copy/pasting, it could bebetter to construct your own tables that also present your own analyses. It is up to you how youchoose to present the data and your analyses. The lab’s normal range for CH50 is 43-90 U/ml.Table 1: CH50 assay raw dataCH50 assays were performed on dilutions of serum taken from healthy control, Patient X and PatientY. Results are represented as Absorbance at 541nm indicates the extent of haemolysis in theexperimental samples. For further details of the assay set-up see the Protocol on BREO.0.00 0.10 0.15 0.20 0.30 0.400.00(0.40 mlNH3)A 0.0463 0.1200 0.4438 1.2313 1.6563 1.7438 1.7450B 0.0377 0.1183 0.4667 1.2974 1.7251 1.8083 1.8122A 0.0324 0.0468 0.0480 0.0504 0.0540 0.0648 1.7472B 0.0377 0.0533 0.0533 0.0533 0.0546 0.0637 1.8772A 0.0450 0.0500 0.1300 0.3238 0.8730 1.0365 1.8825B 0.0390 0.0455 0.1417 0.3354 0.8558 1.0440 2.0072Serum sample volume of serum added to assay (ml)ReferenceSerum XSerum YAbsorbance readingsID Replicate