Your team has been given the following gene with the sequence. This gene consists of three exons (upper case) and two introns (lower case). A messenger RNA is generated from the complementary strand. (Template strand). The mRNA will consist of noncoding regions and a coding region.
5’ -ATG AAG gta tag cag GTT TTA gta cag TAG-3’
Please write the sequence of the complementary strand from 3’ to 5’
Please write the sequence of the mRNA after the introns have been removed assuming normal splicing occurs and the entire DNA fragment is transcribed. Indicate the noncoding regions and the coding region.
Please use the codon table to translate the mRNA using 3-letter designations of the amino acids from the N-terminus to the C-terminus.
Please calculate the net charge at
Please write the sequence of the mRNA if the first intron is left in the final mRNA product while the second one is spliced out and all the exons are in
Please write the sequence of the protein product of the mRNA in #5 from N to C terminus.
PH1=
PH14=
PH7.4=
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