View the following videos:
Enzyme immunoassay (EIA) to detect antigens – Multi-Lingual Captions:
Enzyme-Linked Immunosorbent Assay (ELISA) to Measure Specific Serum Antibodies:
Describe the steps involved in performing each of the following tests (see questions).
1. Test cellular material collected from the lesions of a patient for the presence of varicella zoster virus (VZV) antigens by direct immunofluorescence.
2. Test cellular material collected from the lesions of a patient for the presence of varicella zoster virus (VZV) antigens by indirect immunofluorescence.
3. Test a patient’s serum for the presence of antibodies against Treponema pallidum (the bacterium that causes syphilis) by immunofluorescence.
4. Use an ELISA (enzyme-linked immunosorbent assay) [or EIA (enzyme immunoassay)] to test a patient’s serum for the presence of antibodies againstmeasles virus..
5. Use an ELISA (enzyme-linked immunosorbent assay) [or EIA (enayme immunoassay)] to test a sick animal’s serum for the presence of measles virus antigens.
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